Management of the breeding environment for senescence mice and their background data

高橋 英機

研究基盤開発部門 動物資源開発支援ユニット


Mice used in aging research are kept without pathogenic contamination over a long period of time. It is desirable that mice are ready to be used for researches at appropriate time with the background data of the animals. Specific pathogen free (SPF) animals are mainly used for researches. Recently, there has been an increasing interest in germ-free (GF) mice among researchers. The goal of this study is to provide high-quality aged mice in a systematic way and supplying their body weight, survival, and home cage activity data which are the background data for animal research. Research Resources Center (RRC) has maintained SPF animal by using Individually Ventilated Cage (IVC) system and by performing periodical microbiological test on the mice produced by genetically modification method, by in vitro fertilization (IVF) method and intracytoplasmic sperm injection (ICSI)/round spermatid injection (ROSI) method, by backcrossing with certain genetic background mice. To maintain GF animals, all of GF animals will be introduced by hysterectomy method and be kept in the sealed positive pressure (SPP) IVC system with a HEPA filter within the cage and periodical microbiological test will be performed. Although most commonly used method of GF animal caging system is vinyl isolator, due to the developing technology, IVC system might become more common for GF animal caging system. By preparing and providing a quality research support system, I can expect more active research and maximizing the return to society.